cQC - cDNA Quality ControlAdvanced Submission Form   

A tool for resolving putative sequencing errors in single-pass cDNA, based on genomic sequence

Application Publication Cleaned Sequences
Source code for cQC.
Documentation
Please give us your feedback.
Link to the manuscript (as seen in Bioinformatics)
Link to the supplementary text
Link to the supplementary tables
Link to the supplementary figures

Rice
  NIAS/FAIS/RIKEN consortium
Arabidopsis
  RIKEN
  Genoscope


Web-based cQC (advanced form)

Click here for Basic Submission Form

1
Choose local file containing plain text, FASTA formatted cDNA sequences (no more than 1000 sequences, please):
2
Genome:
3 Enter e-mail address :
(since processing may take a while, we will e-mail you the results)
4 Select all parameters:


MegaBlast E-value cutoff for locating genomic sequence that is similar to a given cDNA sequence:
 
Percent ID cut-off:
 

Maximum intron size (bp)
 

Output files:
Cleaned Sequences
 
Altered cDNA Frequencies
 
Alignment of Altered cDNAs
 
No Genomic Counterpart
 
Lacking End Similarity
 
Lacking Internal Similarity
 
Chimeric Sequences
 
rDNA-Containing Sequences
 
IS-Containing Sequences
 



5
Note: Depending on number of submitted sequences and server load, this may take anywhere from a few seconds to a few hours.

 

cQC website is hosted by the Biotechnology Computing Facility (BCF)
http://bcf.arl.arizona.edu